BACTERIAL OPTIMIZATION OF BIODETERGENT SYNTHESIS AND LIPOLYTIC ACTIVITY INDUCED BY WASTE RESIDUAL OIL

• DOI: 10.37572/EdArt_3107239109

• Palavras-chave: Soil, heterotrophic aerobic bacteria, emulsification, hydrolytic enzimes, hydrocarbons metabolism.

• Keywords: Soil, heterotrophic aerobic bacteria, emulsification, hydrolytic enzimes, hydrocarbons metabolism.

• Abstract:

  In soil contaminated by waste motor oil. It´s possible to isolate genera and species of bacteria with the capacity to synthesize biodetergents and lipolytic activity to emulsify and hydrolyze WMO. Of interesting potential value for use in the bioremediation of environments impacted by hydrocarbons. The objectives of this work were: The objectives of this work were: 1) to analyze that WMO and lubricating oil are inducers of biodetergent synthesis and lipolytic activity 2) to optimize the synthesis of crude biodetergents and lipolytic activity in these genera and bacterial species 3) to partially characterize the type of biodetergent synthesized by this bacterial group. In this sense, Achromobacter denitrificans, Bacillus horneckiae, B. safensis, B. subtilis and Gordonia amicalis isolated from soil impacted by WMO were inoculated in a mineral medium with lubricating oil and WMO as a carbon source, cultivated at 30°C and 200 rpm for 144 h compared to Xanthobacter autotrophicus. To detect and partially characterize anionic biodetergents and lipolytic activity, in a mineral medium with WMO. The optimization of the synthesis of biodetergents and lipolytic activity were used two sources of inorganic nitrogen NH4NO3, and NaNO3 under agitation. For which, every 48 h / 6 days, the anionic glycolipid biodetergents, their emulsification capacity, lipolytic activity and quantification of WMO consumption as an inducer of biodetergents and lipolytic activity were detected in the crude extracts, all experimental data were analyzed by ANOVA Tukey. The results showed that A. denitrificans reached the highest lipolytic activity of 280.3 U/mL in mineral medium with WMO and 210.07 3 U/mL in mineral medium with lubricating oil. Compared with X. autotrophicus, that reached 275.53 U/mL induced by WMO and 165.6 U/mL induced by lubricating oil. G. amicalis reached the maximum production of anionic biodetergent with 1.13 ml/100 ml of mineral medium and WMO. The WMO was the best inducer for the optimization of biodetergent synthesis and lipolytic activity, thus in A. denitrificans with NaNO3, at pH 7 and 250 rpm reached 0.3147 mg of crude biodetergents/mL, and 0.654 mL of anionics/100 mL of mineral medium compared to X. autotrophicus that induced by WMO, NaNO3, pH 6.5 and 250 rpm registered the highest concentration of crude biodetergent with 0.352 mg/mL, 0.7156 mL of anionic biodetergent/100 mL of mineral medium at 144 h of incubation. A. denitrificans induced WMO with NaNO3, pH 6.5 and 250 rpm reached 94.23 U/ml. 197.63 U/mL of lipolytic activity compared to X. autotrophicus with and 45.16 U/mL of lipolytic activity. The highest emulsification rate of 100% was registered in the crude biodetergents of A. denitrificans with NaNO3, at pH 6 and 250 rpm. Qualitatively, the glycolipids were the most synthesized for those genera. The WMO with NH4NO3 pH 6, 250 rpm, was the greatest inducer of biodetergents synthesis and lipolytic activity by A. denitrificans. This research showed the potential capacity of genera and species isolated from soil impacted by WMO to synthesize biodetergents and induction of lipolytic activity for an efficient application in the bioremediation of soil impacted by hydrocarbon mixtures. 

• Número de páginas: 25